Calling conventions refers to the specifications that the two functions should follow when one function calls another function. For example, how to pass parameters and a return value between them. Calling conventions are part of the application binary interface (ABI).
After the x86 PC boots, it will be in real mode. At this time, we can access memory below 1 MB. However, the BIOS also uses some memory. Therefore, we must know which areas the BIOS occupies in order to avoid them.